Tenon’s fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, without influencing cell size/granularity. Upregulation of EGF, VEGF, KDR, MMP2‑9, FAK mRNA levels also occurs and phosphorylation of AKT but not of ERK1/2 is significantly enhanced. The inhibition of the PI3kinase/AKT pathway with the specific inhibitor wortmannin, decreases PL-induced cell migration but not proliferation. Condition supernatants containing PL show increased bioavailability of Nitric Oxide and reduced levels of 8-Iso-PGF2-alpha, correlating with cell proliferation and migration. Pro-angiogenic/inflammatory soluble factors (GRO, Angiogenin, EGF, I-309, PARC) are exclusively or greater expressed in media containing PL than FBS. GMP-grade PL preparations positively influence in vitro biological effects of TFs representing a suitable and safer alternative to FBS.

GMP-grade platelet lysate enhances in vitro Tenon’s fibroblast proliferation and migration correlating with oxidative stress levels / Augusto, Carducci; Scafetta, Gaia; Siciliano, Camilla; Carnevale, Roberto; Rosa, Paolo; Coccia, Andrea; Mangino, Giorgio; Bordin, Antonella; Vingolo, Enzo Maria; Pierelli, Luca; Lendaro, Eugenio; Ragona, Giuseppe; Frati, Giacomo; DE FALCO, Elena. - In: FRONTIERS IN BIOSCIENCE. - ISSN 1945-0516. - ELETTRONICO. - 8:1(2016), pp. 84-89. [doi.org/10.2741/753]

GMP-grade platelet lysate enhances in vitro Tenon’s fibroblast proliferation and migration correlating with oxidative stress levels

SCAFETTA, GAIA
Secondo
;
SICILIANO, CAMILLA;CARNEVALE, Roberto;ROSA, PAOLO;COCCIA, ANDREA;MANGINO, GIORGIO;BORDIN, ANTONELLA;VINGOLO, Enzo Maria;PIERELLI, LUCA;LENDARO, Eugenio;RAGONA, Giuseppe;FRATI, GIACOMO
Penultimo
;
DE FALCO, ELENA
Ultimo
2016

Abstract

Tenon’s fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, without influencing cell size/granularity. Upregulation of EGF, VEGF, KDR, MMP2‑9, FAK mRNA levels also occurs and phosphorylation of AKT but not of ERK1/2 is significantly enhanced. The inhibition of the PI3kinase/AKT pathway with the specific inhibitor wortmannin, decreases PL-induced cell migration but not proliferation. Condition supernatants containing PL show increased bioavailability of Nitric Oxide and reduced levels of 8-Iso-PGF2-alpha, correlating with cell proliferation and migration. Pro-angiogenic/inflammatory soluble factors (GRO, Angiogenin, EGF, I-309, PARC) are exclusively or greater expressed in media containing PL than FBS. GMP-grade PL preparations positively influence in vitro biological effects of TFs representing a suitable and safer alternative to FBS.
2016
Platelet lysate, mesenchymal stem cells
01 Pubblicazione su rivista::01a Articolo in rivista
GMP-grade platelet lysate enhances in vitro Tenon’s fibroblast proliferation and migration correlating with oxidative stress levels / Augusto, Carducci; Scafetta, Gaia; Siciliano, Camilla; Carnevale, Roberto; Rosa, Paolo; Coccia, Andrea; Mangino, Giorgio; Bordin, Antonella; Vingolo, Enzo Maria; Pierelli, Luca; Lendaro, Eugenio; Ragona, Giuseppe; Frati, Giacomo; DE FALCO, Elena. - In: FRONTIERS IN BIOSCIENCE. - ISSN 1945-0516. - ELETTRONICO. - 8:1(2016), pp. 84-89. [doi.org/10.2741/753]
File allegati a questo prodotto
File Dimensione Formato  
Carducci_GMP-grade-platelet_2016.pdf

solo gestori archivio

Note: Articolo PDF
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.67 MB
Formato Adobe PDF
2.67 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/783008
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 14
  • ???jsp.display-item.citation.isi??? ND
social impact